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Similarities and differences between real-time fluorescent quantitative PCR and ordinary PCR
- Categories:Medical News
- Time of issue:2022-09-11
(Summary description)
Similarities and differences between real-time fluorescent quantitative PCR and ordinary PCR
(Summary description)
- Categories:Medical News
- Time of issue:2022-09-11 16:00
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Similarities and differences between real-time fluorescent quantitative PCR and ordinary PCR
Similarities:
The results of the two are the same, which can explain the amplification efficiency and dissolution temperature of the whole process of special DNA replication
in vitro.
Difference:
1. The two systems have different composition
Fluorescence quantitative PCR instrument has more fluorescence signal acquisition system and computer analysis and processing system than ordinary PCR instrument.
2. The principle of the two is different
Fluorescence quantitative PCR monitors the fluorescence excited by the fluorescent dye bound to DNA in real time, and ordinary PCR measures the amount of the final product of PCR by detecting the amount of the dye inserted into the DNA.
3. The response requirements of the two are different
Fluorescence quantitative PCR has higher requirements for amplifying fragments, generally 100-300bp, and ordinary PCR can amplify long fragments.
4, the two applications are different
Fluorescence quantitative PCR is mainly used to quantitatively analyze and determine the level of gene transcription. Ordinary PCR instruments are used for qualitative analysis and amplification of gene fragments. Quantitative PCR instruments can do the work of ordinary PCR instruments, but not vice versa.
5. The measurement cost of the two is different
Quantitative PCR instruments are relatively expensive, and ordinary gene amplification instruments (PCR instruments) can only qualitatively analyze the existence of target fragments. But the price is much lower, and the running cost is much lower.
Real-time fluorescence quantitative PCR technology effectively solves the limitation of traditional quantitative only end-point detection, realizes the detection of the intensity of the fluorescent signal once in each cycle, and records it in the computer software, through the calculation of the Ct value of each sample, Quantitative results were obtained according to a standard curve.
Some real-time quantitative PCR products sold by FKNMED can benchmark with similar products of ThermoFisher Scientific. For specific product information, please refer to https://www.fknmed.com/product/Molecular-Diagnosis-134
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